Comparison of TAE and TBE electrophoresis buffer

Properties TBE TAE Remarks
Buffering capacity High Low TAE become exhausted during extended or repeated electrophoresis
Migration of (double stranded) DNA Slow Fast TAE has better conductivity
Resolution High resolution long DNA fragments High resolution for Short DNA fragments TBE supports agarose cross-linking better than TAE
Enzymatic modification of gel purified DNA Poor Good Borate from TBE buffer is an inhibitor of many commonly used enzymes in molecular biology
Recovery of DNA from agarose gel Poor Good Borate from TBE buffer interacts with DNA
Integrity of DNA High Low Borate from TBE buffer inhibits many enzyme activity including DNA modifying enzymes
Cost Costly Cheaper Highly conc. TAE (50X) require less volume to be transported.
Active buffer Tris – Acetate Tris – Borate
Working conc for DNA electrophoresis 1X 0.5X