Preparation of stock solution of streptomycin

Overview

  • Streptomycin function by inhibiting protein synthesis in bacteria.
  • Binding of streptomycin to the small 16S rRNA of the 30S subunit of the bacterial ribosome subunit prevent access of aminoacyl tRNA to the acceptor site on the mRNA – ribosome complex, leading to inhibition of protein synthesis.
  • It binds to the small 16S rRNA of the 30S subunit of the bacterial ribosome, interfering with the binding of formylmethionyl-tRNA to the 30S subunit.

Requirement

  • Reagents
    • streptomycin
    • Deionized / Milli-Q water
  • Equipment and disposables
    • Measuring cylinder
    • Conical flask / Beaker / 15 ml screw-capped tube
    • Magnetic stirrer

Composition

  • 50 mg/ml streptomycin

Objective

  • Preparation of 10 ml of 50 mg/ml streptomycin stock solution in water

Preparation

Step 1: To prepare 10 ml of stock solution of streptomycin, weigh out 100 mg of streptomycin. Transfer it to 15 ml screw-capped tube ml / beaker / conical flask. Add 9 ml of deionized / Milli-Q water. Mix until all streptomycin is dissolved completely.

Tips:

  • We recommend to use 15 ml screw-capped tube with millilitre marks. Transfer all the contents in it. Add 9 ml deionized / Milli-Q water and mix the content by rotating the tube. Once the content is mixed, make up the volume 10 ml.

Precautions:

  • Do not dissolve in 10 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute dissolves in solvent.

Step 2: Adjust the volume to 10 ml with deionized / Milli-Q water. Mix it again.

Storage

  • Store the solution in small aliquots (1 ml) at -20°C for long time.

Note:

  • It is recommended to store the tetracycline stock solution in small aliquots.

Applications

  • A selection marker for bacterial culture during gene cloning experiments.

 

Follow the table to prepare streptomycin solution of various volume (5 ml, 10 ml, 25 ml, 100).
Reagents / Volume 5 ml 10 ml 25 ml 100 ml
streptomycin 50 mg 100 mg 250 mg 1000 mg
Deionized / Milli-Q water Adjust the final volume to 5 ml Adjust the final volume to 10 ml Adjust the final volume to 25 ml Adjust the final volume to 100 ml