Preparation of 10% (w/v) Sodium dodecyl sulfate (SDS) solution

Overview

  • Sodium dodecyl sulfate (SDS), also known as Sodium lauryl sulfate (SLS), is an anionic surfactant (detergent).
  • It is extensively used in biological research as a strong protein denaturant and lipid-solubilizing agent.
  • It is also used in our cleaning and personal care products, e.g., shampoos, hand soaps, bath products, shaving creams, toothpaste.
  • SDS is water soluble, white to yellowish powder.
  • Heating dissolves SDS quickly in water, thus helpful to prepare concentrated SDS aqueous solution (20% SDS solution in water). Once dissolved, SDS forms clear transparent or slight yellowish solution.
  • 10% or 20% (w/v) stock solution of Sodium dodecyl sulfate (SDS) is generally prepared in distilled water (or milli Q water).

Requirement

  • Reagents
    • Sodium dodecyl sulfate (CH3(CH2)11OSO3Na, Molecular weight: 288.38, CAS Number: 151-21-3)
    • Deionized / Milli-Q water
  • Equipment and disposables
    • Measuring cylinder
    • Conical flask / Beaker
    • Magnetic stirrer with heat control (optional)

Composition

  • 10 % Sodium dodecyl sulfate

Objective

  • Preparation of 100 ml of 10 % SDS in Deionized / Milli-Q water.

Preparation

Step 1: To prepare 100 ml of 10 % SDS solution, weigh out 10 grams SDS in 250 ml conical flask / beaker. Add 80 ml deionized / Milli-Q water and mix it.

Tips:

  • One can use manual shaking using a glass pipette to mix the ingredients. Magnetic stirrer makes the dissolving process easy and convenient.

Precautions:

  • Do not dissolve in 100 ml of deionized / Milli-Q water. In most cases, solution volume increases when the large amount of solute is dissolved in solvent.
  • Wear face mask to avoid inhalation of SDS dust.
  • Try to avoid frothing.

Step 2: Heat to 68 °C if necessary.

Tips:

  • One can set the magnetic stirrer temperature 68 °C. If magnetic stirrer is unavailable, place the solution in a water bath set at 68 °C.

Precautions:

  • Make sure to dissolve any residual powder sticking to the glass.

Step 3: Adjust the volume to 100 ml with deionized / Milli-Q water. Mix it again.

Step 4 (Optional) : One can filter the solution to remove any undissolved material.

Notes:

  • Do not autoclave 10% SDS solution.

Storage

  • Solution can be stored at room temperature for several months.

Precautions:

  • Do not store the solution at 4°C. SDS will precipitate at temperature below 15°C. If there is any precipitate, redissolve by warming the solution (68°C for 10 min)

Applications

  • Plasmid isolation by alkaline lysis method
  • SDS-PAGE
  • Preparation of Laemmli sample buffer
  • Stripping of immunoblot
Follow the table to prepare Sodium dodecyl sulfate (SDS) solution of various volume (10 ml, 100 ml, 500 ml and 1,000 ml) and percentage (1%, 5%, 10% and 20%).
Reagents / Volume 10 ml 100 ml 500 ml 1000 ml
1% 0.1 g 1.00 g 5.00 g 10.00 g
5% 0.50 g 5.00 g 25.00 g 50.00 g
10% 1.00 g 10.00 g 50.00 g 100.00 g
20% 2.00 g 20.00 g 100.00 g

200.00 g