Monthly Archives: September 2016

Agarose

  • Research in past three decades has established agarose as a useful matrix for number of biochemical techniques including gel electrophoresis, chromatography and support matrix to immobilize enzymes and cells.
  • These techniques utilize agarose for various molecular biology and biochemistry applications including nucleic acid analysis and purification, protein analysis and purification, immunodiffusion, gel filtration of viruses and sub-cellular particles, etc.
  • In addition, agarose can be used to culture cells. It can also be used in place of agar to prepare bacterial culture medium and also for animal cell culture (e.g., soft agar assay).
  • Agarose is available in a variety of form, which differ in physical properties.
  • Suitability of agarose for an application depends on its properties. The following parameters are considered while choosing agarose for an application:
    • Purity of agarose (mostly defined as the presence of sulfate content)
    • b. Melting/gelling point of agarose
    • c. Gel strength of gel
    • d. Electroendosmosis (EEO)
  • Lower grade agarose may be contaminated with other polysaccharides, as well as salts and proteins and often have high EEO, making them unsuitable for sensitive assays.
  • Although, ultra purified agarose are better choice for most assay, but they are costly which restrict their use.
  • Often the quality of agarose, their suitability for different assay are described in suppliers catalog, which can be used as a reference to chose an agarose for a particular application.